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Invitrogen原装R25001 Zeocin 博莱霉素

发表时间:2015-02-04  |  点击率:1603

 Invitrogen R25001 Zeocin 博莱霉素

Bleomycin、Bleocin,BLM

CAS  1056-06-7

争光霉素、博来霉素、硫酸博来霉素、硫酸博莱霉素


货 号

品      名

产地

规格

单位

价格

R25001

zeocin 
   博莱霉素

Invitrogen

125mg/1.25mL

450

R25001

zeocin 
   博莱霉素

Invitrogen

8*1.25mL /1g

2250

R25005

zeocin 
   博莱霉素

Invitrogen

50mL /5g

9500


Zeocin? 试剂是博来霉素抗生素家族的一个成员。 其抗性由编码一种 13,665 道尔顿蛋白质的 Sh ble 基因赋予。 在表达这种蛋白质的细胞中,Zeocin? 无法结合和切割细胞 DNA。 Zeocin? 对大多数好氧细胞有效,可用于选择哺乳动物和昆虫细胞系、酵母以及细菌。 选择细胞时,Zeocin? 的用量为 50-2000 ug/ml(通常 300 ug/ml),具体取决于细胞类型。

化学式:C55H83N19O21S2Cu    式量:1137.41 g/mol

Zeocin可选择Shble基因表达的细胞。与现在使用的其他动物细胞标记物无交叉抗性。因此这种抗生素可用来分离对其他筛选剂(如:庆大霉素,潮霉素)有抗性的克隆。Zeocin是一种属于争光霉素家族的糖蛋白抗生素,在体内能作用于大多数细菌(包括E。coli)、真菌(如:酵母菌)、植物细胞、动物细胞、细胞培养级。

使用:
●液体Zeocin可用于细胞培养。
●用于筛选转化株的Zeocin浓度根据pH值和盐浓度改变。
●pH越高,盐浓度越低Zeocin活性越大。
●普通大肠杆菌菌株筛选可在25µg/ml zeocin浓度的低盐LB琼脂培养基中进行,pH调至7.5。
●动物细胞筛选:根据不同细胞系从100到750µg/ml。
●植物细胞筛选:根据不同的细胞种类和培养基从50到250µg/ml。
●酵母筛选:取决于种属和培养基,S。cerevisiae Sh ble转化株在50µg/ml浓度的YPD培养基中

Zeocin? Selection Reagent
Cat. No. R250-01
Description
Zeocin? Reagent is a member of the bleomycin antibiotic family. Resistance is conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cells expressing the protein, Zeocin? is prevented from binding and cleaving the cellular

 DNA. Zeocin? is effective on most aerobic cells and can be used for selection in mammalian and insect cell lines, yeast, and bacteria. The amount of Zeocin? required for selection will vary between 50-2000 ug/ml (typically 300 ug/ml) depending on the cell type.
C55H83N19O21S2Cu
Formula Weight: 1137.41 g/mole
Zeocin? is a trademark of CAYLA.
The ble resistance gene is the subject of one or more of U.S. Patent Nos. 5,021,344 and 5,118,620 and foreign equivalents owned by Cayla and licensed to Invitrogen. This product is sold for research purposes only.

For commercial license information, please contact: Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008.
General Specifications
Concentration:       100 mg?mL
Brand:       Zeocin?
Form:       Liquid
Product Size:      125mg
Validated Application:       Eukaryotic Selection?Stable Cell Line Generation
Bacterial Selection
Contents & Storage
Zeocin? is provided

Invitrogen R25001 Zeocin 博莱霉素

BleomycinBleocinBLM

争光霉素、博来霉素、硫酸博来霉素、硫酸博莱霉素 


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