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sigma弗氏佐剂 火热现货促销中 f5506 f5881
点击次数:3739 发布时间:2012-02-07
 
上海索宝生物科技有限公司 现货 特价 8折 促销
弗氏完全佐剂
Freund’s Adjuvant, Complete
F5881 -cell suspension
F5881-10ML  363.87
F5881-6X10ML  1,123.20
F5881-10X10M  1,763.19
 
General description
每毫升含 1mg 热灭活的干燥结核分枝杆菌 (H37Ra, ATCC 25177)、0.85mL 石蜡油和 0.15mL 二缩甘露醇一油酸。
Specificity
弗氏佐剂可用于制备免疫原的油包水乳液。由于抗原释放缓慢,油包水乳液中的抗原可刺激长期的抗体应答。
Application
通常将抗原(优选地溶于盐水中)与佐剂等体积地混合,以形成乳液。关于方法和剂量信息,请参阅 Harlow, E. and Lane, D., Antibodies a Laboratory Manual, (Cold Spring Harbor Laboratory, 1988)。
Biochem/physiol Actions
弗氏完全佐剂中的分枝杆菌可将巨噬细胞和其他细胞吸引到注射部位,从而增强免疫应答。鉴于此原因,将弗氏完全佐剂用于初始注射,而将弗氏不完全佐剂用于随后的加强注射。
 

弗氏不完全佐剂
Freund’s Adjuvant, Imcomplete
F5506 – liquid
F5506-10ML  333.45
F5506-6X10ML  941.85
F5506-10X10M  1,533.87
 
描述  使用 方法 说明
Biochem/physiol Actions
弗氏佐剂可用于制备免疫原的油包水乳液。由于抗原释放缓慢,油包水乳液中的抗原可刺激长期的抗体应答。
Other Notes
每毫升含 0.85mL 石蜡油和 0.15mL 二缩甘露醇一油酸。
Application

不含存在于弗氏完全佐剂中的分枝杆菌,因此使副作用降至低。鉴于此原因,将弗氏不完全佐剂用于加强注射。
 
FREUND’S ADJUVANT, COMPLETE AND INCOMPLETE
Product Number F 5881 AND F 5506   Storage Temperature 2-8 °C
Product Description
Appearance
F 5881 Clear amber liquid containing particulate matter (dried cells)
F 5506 Clear amber liquid
Freund’s Adjuvant is one of the most commonly used adjuvants in research. It is used as a water-in-oil emulsion. It is prepared from non-metabolizable oils(paraffin oil and mannide monooleate). If it also contains killed Mycobacterium tuberculosis it is known as Complete Freund’s Adjuvant. Without the bacteria it is Incomplete Freund’s Adjuvant. First developed by
Jules Freund in the 1940’s, Freund’s Adjuvant is designed to provide continuous release of antigens necessary for stimulating a strong, persistent immune response1,2,3 The main disadvantage of Freund’s Adjuvant is that it can cause granulomas, inflammation at the inoculation site and lesions. The mycobacteria in Complete Freund’s attracts macrophages and other cells to the injection site which enhances the immuneresponse. For this reason, the Complete Freund’s Adjuvant is used for the initial injections. To minimize side-effects, ncomplete Freund’s Adjuvant is used for the boosts.
For comparisons of different adjuvant systems, see references 4 and 5.
Reagents
Each ml of F 5881 contains 1 mg of heat-killed and dried Mycobacterium tuberculosis (strain H37Ra, ATCC 25177), 0.85 ml paraffin oil and 0.15 ml of mannidemonooleate.
Each ml of F 5506 contains 0.85 ml of paraffin oil and 0.15 ml of mannide monooleate.
Precautions and Disclaimer Please consult the Material Safety Data Sheet for handling recommendations before working with this material.
Storage/Stability
Store in a cooler at 2-8 °C. Do not Freeze.
Procedure
1. If using Complete Freund’s Adjuvant, vortex or shake to resuspend the Mycobacterium.
2. Mix antigens (preferably in saline) with an equal volume of the adjuvant to form an emulsion. In order to do this, vigorous and prolonged mixing is needed. There are at least three methods which can be used to accomplish this:For small volumes the emulsion can be made in a
tube. Pipet the adjuvant in the tube first. Then,while vortexing, add an equal volume of the antigen solution. Vortex vigorously until a thick emulsion forms.For intermediate volumes, use two syringes connected through a luer fitting. Ideally, a 3-way valve should be used. Take the desired amount of antigen solution into a glass syringe. The volume should not fill more than half the syringe. Take an equal volume of the adjuvant into another glass syringe. Remove all air and connect the syringes through the luer fitting to the 3-way valve. Adjust the 3-way valve such that the connection is open between the two syringes. Carefully depress the plunger from the antigen solution first, pushing the antigen into the oil of the adjuvant. Alternay push
the plungers, mixing the adjuvant and the antigen solution into an emulsion. Continue until the
plungers are difficult to push. For large volumes, use a tissue homogenizer to make the emulsion. Add the adjuvant to the homogenizer first. Run the homogenizer for a short time to coat the inside with the adjuvant. Add an equal volume of the antigen solution and run until a
thick emulsion forms.
3. The resulting emulsion should be very thick and a drop of it should not disperse if tested by placing on the surface of a saline solution.
4. Transfer the emulsion to a syringe (or remove one syringe from the luer fitting if using the two-syringe method). Remove all the air. Add an appropriay sized needle. The samples are now ready for injection.
References
1. Freund, J. and McDermott, K., Proc. Soc. Exp. Biol.Med., 49, 548-553 (1942)
2. Freund, J., Ann. Rev. Microbiol., 1, 291 (1947)
3. Freund, J., Adv. Tuberc. Res., 7, 130 (1956)
4. Bennett, B. et al., J. Immuno. Meth., 153, 31-40 (1992)
5. Deeb, B.J. et al., J. Immuno. Meth., 152, 105-113 (1992)
6. Harlow, E. and Lane, D., Antibodies A Laboratory Manual, (Cold Spring Harbor Laboratory, 1988) alc 8/4/98
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip.

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