产品编号 11585614910 | ROCHE
DIG-High Prime DNA Labeling and Detection Starter Kit II
|1.1746E+10||DIG High Prime Lab/Detection Kit I 地高辛高效DNA标记及检测试剂盒I型（底物显色法）||roche||50T||盒||3300|
|1.1586E+10||DIG High Prime Lab./Det.Kit II 地高辛高效DNA标记及检测试剂盒II型（化学发光法）||roche||50T||盒||3450|
辛高素标记的探针，可用于在南方，北方和点杂交分析，以及殖民地和菌斑杂交。该套件提供了与DIG-11-dUTP标记的随机引物标记的DNA模板，碱不稳定，颜色DIG标记的杂交检测。本试剂盒组装方便考虑，提供一个现成的封闭液，NBT / BCIP联合原液，挖易HYB颗粒。挖总理混合物，包括稳定Klenow酶，核苷酸，引物，反应缓冲液，在一个方便的试剂。
灵敏度和特异性：该标准的检测采用线性pBR328 1微克。这是通常的期望打成0.8微克DNA标签后1小时，2.3微克的DNA标记后20小时的反应。0.1同源的DNA PG在斑点杂交，检测到后16小时的色彩发展（探针浓度为20毫微克/毫升）。一个单拷贝人类基因（TPA）是在Southern杂交检测，装载1微克消化胎盘DNA
5. NBT / BCIP联合解决方案，浓，6×1毫升
DIG-High Prime is used for the highly efficient random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled probes are generated at high yield within one hour or after overnight incubation. DIG-High Prime labeled DNA probes are used in a variety of hybridization techniques: • Southern blots
• Northern blots
• Dot blots
• Colony and plaque hybridizations
• For all types of filter hybridization
• For single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat
Convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
1 kit containing 7 components.
The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.
|Related Categories||12 Principles Aligned Products, Chemical Synthesis,Greener Alternative Products, Life Sciences|
|usage ||sufficient for 12 labeling reactions (10 ng to 3 μg per assay)|
| ||sufficient for 24 blots (blots of 100 cm2)|
|mfr. no. ||Roche|
|greener alternative product characteristics ||Designing Safer Chemicals|
Learn more about the Principles of Green Chemistry.
|shipped in ||dry ice|
|storage temp. ||−20°C|
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