储存条件：Upon receipt, store the dye frozen at -20°C, protected from light in a desiccator. When stored properly, the SYBR Green II stain stock solution in DMSO is stable for six months to one year.
产品特点：
SYBR ® Green II RNA gel stain is one of the most sensitive dyes known for detecting RNA in electrophoretic gels. As little as 100 pg RNA or single-stranded DNA per band can be detected in a SYBR Green IIBstained agarose or polyacrylamide gel using 254 nm epi-illumination Polaroid ® 667, black-and-white print film and a SYBR Green gel stain photographic filter. Even with 300 nm transillumination, as little as 500 pg RNA per band can be detected. SYBR Green II RNA gel stain is significantly more sensitive than ethidium bromide, the most commonly used stain for detecting nucleic acids in gels (Figure 1). With 300 nm transillumination and photography through an orange-red gelatin filter, ethidium bromide’s sensitivity limit in a standard agarose minigel is about 1.5 ng single-stranded nucleic acid per band. Note that our detection limits are based on results obtained with a FotoDyne ® Foto/UV ® 450 ultraviolet transilluminator in combination with Polaroid 667 film. Video cameras and CCD cameras in general have a different spectral response than black-and-white print film and thus may not exhibit the same sensitivity.
