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YARIV REAGENTS FOR DETECTION AND QUANTITATION OFAR
描述:YARIV REAGENTS FOR DETECTION AND QUANTITATION OFARThe -glucosyl Yariv reagent can be used as A histochemical reagent to detect AGP's intissue sections (Ande
YARIV REAGENTS FOR DETECTION AND QUANTITATION OFARThe -glucosyl Yariv reagent can be used as
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YARIV REAGENTS FOR DETECTION AND QUANTITATION OFAR
METHOD OF USE As a histochemical reagent: Dissolve 2mg of ?-glucosyl Yariv reagent in 1mL of 0.15M NaC1. Apply to tissue section for 1h at room temperature and examine by bright field microscopy. The AGP's precipitate in the Yariv reagent to give a red stain. The exact colour varies from brown-red to bright red depending on the tissue. As AGP's are usually water-soluble, they may be lost during the procedures for tissue embedding; it is therefore advisable to start with hand or cryostat sections. As a control the ?-Gal Yariv reagent is used. For quantification:
Prepare a solution containing 1% w/v agarose, 0.15M NaC1, 0.02% w/v NaN3 and 10?g/mL ?-glucosyl Yariv reagent. Heat to boiling, pour 3.5mL aliquots onto preheated glass plates (5x7cm); cool. Punch wells in gel formed. Place test solutions in wells; construct standard curve using reference AGP (e.g. gum arabic) according to Van Holst & Clarke (1985). For crossed electrophoresis: Prepare a solution containing 1%w/v agarose in 0.025M Tris, 0.2M glycine, 0.02%w/v NaN3 and 30mg/mL ?-glucosyl Yariv reagent. Heat to dissolve agarose; use in crossed electrophoresis experiments according to Van Holst & Clarke (1986). All solutions are stable for at least a year at room temperature. In some applications the ?-D-Mannosyl Yariv reagent can replace ?-D-Glucosyl Yariv reagent. Similarly ?-D-Mannosyl Yariv reagent can replace ?-D-Galactosyl Yariv reagent.