Alexa Fluor® 594 goat anti mouse IgG antibody/pH 7.2
Absorption and fluorescence emission spectra of Alexa Fluor® 594 goat anti–mouse IgG antibody in pH 7.2 buffer.
Description The Alexa Fluor 594 cholera toxin subunit B conjugate is labeled with our bright, photostable, red-fluorescent Alexa Fluor 594 dye (which is spectrally similar to Texas Red dye). The conjugate is derived from nontoxic recombinant B subunit, so it is compley free of the toxic A subunit. Alexa Fluor 594 cholera toxin subunit B conjugate is also available in a unit size of 500 µg (C-22842).
Revised: 02September2003 Product Information Cholera Toxin Subunit B (CT-B) Conjugates Storage upon receipt: ≤ 20°C Desiccate Protect from light Abs/Em: See Table 1 Introduction Table 1. Conjugates of cholera toxin subunit B. Unit Size Label Abs/Em * 100 µg 500 µg Alexa Fluor 488 495/519 C-34775 C-22841 Alexa Fluor 555 555/565 C-34776 C-22843 Alexa Fluor 594 590/617 C-34777 C-22842 Alexa Fluor 647 650/668 C-34778 Biotin NA C-34779 Horseradish peroxidase (HRP) NA C-34780 * Approximate absorption (Abs) and fluorescence emission (Em) maxima, in nm. NA = Not applicable.
Cholera toxin from Vibrio cholerae is comprised of two sub- units, A and B, arranged in an AB5 configuration. The A subunit is an ADP-ribosyltransferase, which disrupts the proper signal- ing of G proteins and eventually leads to dehydration of the cell. 1 The nontoxic B subunit is important to the protein complex as it allows the protein to bind to cellular surfaces via the pentasac- charide chain of ganglioside GM1 . 2 At neutral pH, the 11.4 kDa B subunit exists as a 57 kDa pentamer. 3 The B subunit of cholera toxin (CT-B) has proven to be a powerful tool for retrograde labeling of neurons. 4,5 This tracer has been used in a variety of applications, including tracing of ra forebrain afferents, 6 projections of the parabrachial region 7 and neurons of the urinary bladder wall. 8 More recently, researchers have found that CT-B can be used as a marker for lipid rafts, which are membrane microdomains enriched in cholesterol and sphingolipids. Lipid rafts segregate specific groups of proteins and thereby provide a hub for cellular signaling and protein traf- ficking. 9, 10 Molecular Probes’ CT-B conjugates (Table 1) are made from recombinant cholera toxin subunit B. Because the B-subunit source material is recombinant in origin, it is extremely pure and compley free of the toxic A subunit.
powder in 0.1 mL or 0.5 mL of buffer, for example, phosphate- buffered saline (PBS). With the addition of 2 mM sodium azide, solutions can be stored at 2–6°C for approximay three months. For longer storage, divide the solution into aliquots and freeze at ≤–20°C. The peroxidase conjugates of cholera toxin B are supplied in a unit size of 100 µg. When stored desiccated at –20°C, the lyo- philized powder is stable for at least six months. Solutions of 1.0 mg/mL can be prepared by dissolving the powder in 0.1 mL of PBS, pH 7.2. Store solutions at 2–6°C with the addition of thimerosal to a final concentration of 0.02%. DO NOT USE AZIDE for the HRP-conjugates. For prolonged storage after re- constitution, add glycerol to a final concentration of 50% (v/v), aliquot and store at –20°C. When stored properly, solutions are stable for approximay three months. PROTECT FROM LIGHT. AVOID REPEATED FREEZING AND THAWING OF SOLUTIONS. For the Alexa Fluor and biotin conjugates, the lot-specific degree of labeling (typically 5–10 moles of dye per mole of the B subunit pentamer) is indicated on the product label.
Product List Current prices may be obtained from our Web site or from our Customer Service Department. Cat # Product Name Unit Size C-34775 cholera toxin subunit B (recombinant), Alexa Fluorfi 488 conjugate .......................................................................................... 100 µg C-22841 cholera toxin subunit B (recombinant), Alexa Fluorfi 488 conjugate .......................................................................................... 500 µg C-34776 , Alexa Fluorfi 555 conjugate .......................................................................................... 100 µg C-22843 , Alexa Fluorfi 555 conjugate .......................................................................................... 500 µg C-34777 , Alexa Fluorfi 594 conjugate .......................................................................................... 100 µg C-22842 , Alexa Fluorfi 594 conjugate .......................................................................................... 500 µg C-34778 , Alexa Fluorfi 647 conjugate .......................................................................................... 100 µg C-34779 , biotin-XX conjugate ....................................................................................................... 100 µg C-34780 , horseradish peroxidase conjugate ........................................................ ........................ 100 µg
Materials The biotin- and dye-labeled cholera toxin B subunits are sup- plied in unit sizes of 100 µg and 500 µg. When stored desiccated at ≤–20°C, these products are stable for at least six months. Solutions of 1.0 mg/mL can be prepared by dissolving the
Due to the diversity of applications for the cholera toxin sub- unit B, please consult the primary literature for appropriate work- ng concentrations.