RNAzol®RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent （1） provides higher yield and quality of isolated RNA than previous reagents based on the single-step method （2,3）. RNAzol®RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.
RNAzol®RT separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA. A biological sample is homogenized or lysed in RNAzol®RT. DNA, proteins, polysaccharides and other molecules are precipitated from the homogenate/lysate by the addition of water and removed by centrifugation. The pure RNA is isolated from the resulting supernatant by alcohol precipitation, followed by washing and solubilization.
- The isolation procedure can be completed in less than one hour. The isolated RNA is ready for use in RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.
- RNAzol®RT isolates mRNA and micro RNA in separate fractions. Alternatively, total RNA containing all classes of RNA in a single fraction can be isolated. In addition, RNAzol®RT allows for the sequential isolation of RNA and DNA.
- The RNAzol®RT procedure is performed at room temperature, including centrifugation.